Olea europaea is an economically important tree species native to Mediterranean region. The present study is focused on developing the regeneration protocol and production of true-to-type plants of Olea europaea L. cv Barnea with commercial importance. Olive tree is a long lived evergreen tree but has a long juvenile phase and reaches its full production stage at 25 – 30 years of age. Traditionally, for many years, they have been propagated through cutting, grafting, vegetative propagation and in recent years, through air layering through plant tissue culture methods this problem of long juvenility can be overcome in addition large number of pathogen free plants can be produced. Nodes from adventitious shoots were surface sterilized and inoculated in different tissue culture media such as Woody Plant Media (WPM), Murashige and Skoog media (MS), Rugini Olive media (OM), Schenk and Hildebrandt media (SH) and Multiplication media (Combination of half strength MS and half strength Rugini OM) in which culture establishment was best obtained in multiplication media supplemented with 1 mg/l zeatin. Different treatment was given to induce shoot proliferation and the highest shoot number 4.2±0.4 was obtained in multiplication media enriched with 2.0 mg/l zeatin and silver nitrate at 2.0 mg/l. The in-vitro derived shoots were assessed for genetic fidelity using different molecular markers Random Amplification of Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). Monomorphic bands were obtained between the mother DNA and in-vitro derived progenies which indicated that there is genetic stability in the in-vitro regenerated plants. Commercial analysis was done with respect to Industrial Use. The global market for olive is estimated to grow at a compound annual growth rate of 4.5% in the forecast period of 2019-2024. In the production of olive, Spain holds the largest share of world production and Italy holds the second largest share. Although in recent years, the world has begun to see olive from various new regions. The present study is the initiative step towards propagation of Olive cultivar in South Asian region.
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