Experimentation on the Validation of the Method for Determining Aflatoxin B1 in Groundnut Pastes Sold in the Markets of Abidjan (Côte d’Ivoire)

Experimentation on the Validation of the Method for Determining Aflatoxin B1 in Groundnut Pastes Sold in the Markets of Abidjan (Côte d’Ivoire)


This study was carried out to validate the method of dosage of Aflatoxin B1 in the groundnut pastes
for high performance liquid chromatography, according to the NFV03-110 Mai 2010 standard. The
tests of the validation were performed out starting from ten samples. The linearity of calibration
established between 0 and 3.0 μg/L was proven by determining the square of the coefficient of
correlation of Pearson (R2 = 0.9985). The limits of detection and quantification were respectively of
0.01 µg/kg and 0.02 µg/kg. The coefficients of variation of the tests of repeatability and reproducibility
are respectively 3.4% and 3.79%, lower than 5%. The rate of recovery were estimated at 107.6%.
The test of conformity did not show a significant difference (P ˃ 0,05) between the average
concentration in aflatoxine B1 (2.02 ± 1.44 μg/kg) and that of the certified value (2 μg/kg).
The method of quantifying aflatoxin B1 in peanut paste by using HPLC can be regarded as acceptable for an analysis of routine.

Author (s) Details

Zamblé Bi Irié Abel Boli
Department of Food Science and Technology (UFR-STA), Laboratory of Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire.

Fatoumata Camara
Department of Food Science and Technology (UFR-STA), Laboratory of Nutrition and Food Safety, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire.

Djegba Marie Toka
Department of Food Science and Technology (UFR-STA), Laboratory of Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire.

Marina Koussemon
Department of Food Science and Technology (UFR-STA), Laboratory of Nutrition and Food Safety, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire.

Rose Koffi-Nevry
Department of Food Science and Technology (UFR-STA), Laboratory of Biotechnology and Food Microbiology, University Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/268

Editor 251News

leave a comment

Create Account



Log In Your Account