A fish specimen of total length (5.5 cm) captured from Pharping was instantly photographed and preserved in 95% ethanol. Its morphological characters including morphometric and meristic data were recorded using digital calipers and backlit dissecting scope. The tissue removed from the caudal fin was placed in 95% ethanol and sent to a reputed lab in Kathmandu city for molecular analysis. The CO1 segment of mitochondrial DNA was amplified and sequenced using a cocktail of four fish specific primers. The DNA sequence (607 bp) was input in BLAST to find out highly similar sequences in GenBank. The sequence was uploaded in GenBank to obtain accession number. The external morphology mainly homodont teeth in jaws forming a crescent –shaped band on upper jaw and absence of post labial groove identified the fish specimen as Parachiloglanis hodgarti and it was found to be distantly related with other glyptosternoid fish based on multiple DNA sequence analysis. Based on the BLAST result the fish specimen was found to be homologous with Myersglanis blythi from Mugu, Nepal. The present study concluded that M. blythi from Mugu was most probably P. hodgarti which was misidentified, and further study on distribution and abundance of P. hodgarti and M. blythi was recommended as the peculiar water resources of Pharping was noteworthy for the study of rare species.
Prithvi Narayan Campus, Pokhara, Tribhuvan University, Nepal.
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