Effect of Size of Crowder on Protein Stabilization

Effect of Size of Crowder on Protein Stabilization

There are a large number of biomolecules responsible for the extremely crowded intracellular environment, which, i.e. the idealised conditions, are entirely different from the dilute solutions. Owing to the existence of macromolecules of various sizes, shapes, and composition, such a crowded environment governs the degree of crowding within a cell. The effect of different sizes and shapes of crowders (ficoll 70, dextran 70, and dextran 40), which are polysaccharides in nature, on the thermodynamic stability of two models of UV-Vis spectroscopy proteins has therefore been investigated. We found that due to the excluded volume effect and the small-sized and rod-shaped crowder, i.e., the level of stabilisation of alpha-lactalbumin and lysozyme increases with the increasing concentration of the crowding agents. In addition to dextran 70 and ficoll 70, dextran 40 resulted in greater stability of both proteins. Because different osmolytes have a functional and structural effect on IDPs, they can help to improve the pathology of different human diseases (such as amyloidosis, neurodegeneration, cancer, and diabetes), with the common hallmark being the aggregation of IDPs. The crowders (sugar osmolyte polymers) used in our research may therefore have clinical implications for diseases, including IDPs.

Author(s) Details

Sumra Shahid
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Ikramul Hasan
Department of Basic Medical Science, Faculty of applied Medical Sciences, Al-Baha University, PO Box: 1988- Al-Baha 65411, Kingdom of Saudi Arabia.

Faizan Ahmad
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Md. Imtaiyaz Hassan
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Asimul Islam
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/343

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