Pharmacological Effect of Krameria cytisoides on Trypanosoma cruzi in vitro and in vivo

Pharmacological Effect of Krameria cytisoides on Trypanosoma cruzi in vitro and in vivo

Objetive: The know the pharmacological effect of Krameriacytisoides extract in vitro and in vivo, as
well as the cytotoxicity of this compound, determined.
Materials and Methods: For in vitro determination, concentrations of 40, 80 and 160 mg/kg of
K. cytisoides were used. Cytotoxicity was assessed with VERO cells using concentrations between 10
and 160 µg/L. The pharmacological effect in vivo was investigated with five groups males per groups
3 of Balb/c mice. All mice were inoculated with 1×103/ µL trypomastigotes. Three groups were injected
with 40, 80 or 160 mg/mL. The fourth group was treated with 100 mg/kg of the benznidazole reference
drug, and no treatment was given to the fifth group.
Results: The results demonstrated that the LD50 was 69 µg/mL, with an LC50 cytotoxicity of 160
µg/mL; thus, no toxic effect was observed. Regarding the in vivo results, 61% and 74% flagellates
death was observed at the highest concentration of K. cytisoides, and the reference drug
benznidazole, respectively.
Discussion and Conclusions: According to the results, the low toxicity and lethality of K. cytisoides
compared to benznidazole suggests that the former could be used as a drug of choice for Chagas
disease.

Author(s) Details

E. Jiménez-Cardoso
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez #162; Col. Doctores, C.P. 06720, México.

L. Soto-Quintanar
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez #162; Col. Doctores, C.P. 06720, México.

B. Nogueda-Torres
Laboratorio de Helmintos, Departamento de Parasitología, Escuela Nacional de Ciencias Biológicos-Instituto Politécnico Nacional, Mexico.

M. S. Pérez-Gutiérrez
Universidad Autónoma Metropolitana Unidad Xochimilco, Mexico.

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