Phenotypic and Genotypic Characterization of Thermophilic Campylobacter Isolated from Chicken in Côte d’Ivoire

Phenotypic and Genotypic Characterization of Thermophilic Campylobacter Isolated from Chicken in Côte d’Ivoire

Thermophilic Campylobacters are major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. In Coteˆ d’Ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. The aims of this study were to determine prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from chickens ceaca in commercial slaughter in Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken caeca samples were collected and analyzed by passive filtration method followed by molecular identification (PCR). From these 119 samples, 76 (63.8%) were positive to Campylobacter tests. Among the positive colonies, 51.3% were C. jejuni and 48.7% were C. coli. Of the 39 C. jejuni isolates, 79.5%, 38.5%, 17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid, ciprofloxacin, amoxicillin, erythromycin, and gentamicin. Among the 37 isolates of C. coli, 78.4%, 43.2%, 13.5%, 8.1%, and 0% were resistant, respectively, to the same antibiotics. On the other hand, detection of virulence putative gene shows presence of cadF in 100 % of tested strains. In addition, cdtA, cdtB and cdtC genes were detected in 100%; 69.43% and 71.06% respectively of C. jejuni isolates. Moreover, only cdtA gene of cdt genes was detected in 12.82% of C. coli strains tested in this study. In conclusion, we reported in this study the presence of high Campylobacter contamination of the studied chickens. Molecular identification of the bacteria was performed and determination of high resistance to antimicrobials of the fluoroquinolone family was revealed.

Author(s) Details

Dr. Goualié Gblossi Bernadette
Laboratoire  de Biotechnologies, Agriculture et Valorisation des Ressources  Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.

Dr. Akpa Eric Essoh
Laboratoire  de Biotechnologies, Agriculture et Valorisation des Ressources  Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.

Dr. Kakou-N’Gazoa Elise Solange
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.

Dr. Guessennd Natalie
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire

Dr. Bakayoko Souleymane
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.

Prof. Niamké Lamine Sébastien
Laboratoire  de Biotechnologies, Agriculture et Valorisation des Ressources  Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.

Prof. Dosso Mireille
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.

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