Advanced Study on Assay of Quinine Utilizing Isocratic HPLC Solvent Conditions and Feasibility of Assay from Human Urine

Using isocratic solvent conditions and high-performance liquid chromatography, the assay of quinine sulfate, a medication provided for the clinical treatment of Plasmodium falciparum, can be performed (HPLC). The most serious form of human malaria is caused by Plasmodium falciparum (P. falciparum), which has been found to be responsible for 85 percent of all cases of malaria. Furthermore, this research aims to demonstrate that it is possible to classify and assay quinine from raw human urine. An ultraviolet light detector, which is set at 222 nm, detects the drug quinine after elution (or alternatively at 254 nm). The stock solution of quinine sulfate was prepared in a solvent containing 64% ethanol (v/v) and 36% water (v/v) at a molar concentration of 1,3155 x 10-2. The test samples injected into the HPLC instrument consisted mainly of a solvent consisting of 95% (v/v) water and 5% ethanol (v/v). The column solvent can range from 5% ethanol to 20% ethanol (v/v), with 1% to 4% glacial acetic acid (v/v) and distilled water being the remaining amount. 2.2628 x 10-5 molar was found to be the limit of detection (LOD) and 7.542 x 10-5 molar was found to be the limit of quantitation (LOQ). This highly sensitive technique for testing quinine from water mixtures, 5% glucose, tablets based on cellulose and directly from urine would be useful in assessing patient compliance and maintenance of the regimen, as well as in manufacturing quality control.

Author (s) Details

Dr. Ronald Bartzatt
University of Nebraska at Omaha, Department of Chemistry, 6001 Dodge Street, Omaha Nebraska, 68182, USA.

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