The author describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an Inertsil® ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the analytes. The total run time was < 8 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were <46 ng.mL–1. An inexpensive and harmless method for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further applied to the quantification in foods.
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
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