Chemical Changeability of Essential Oils in Peels of C. reticulata ‘Dahongpao’ by Gas ChromatographyMass Spectrometry Compiled with Automated Mass Spectral Deconvolution & Identification System

Chemical Changeability of Essential Oils in Peels of C. reticulata ‘Dahongpao’ by Gas ChromatographyMass Spectrometry Compiled with Automated Mass Spectral Deconvolution & Identification System

Chemical variability of essential oils (EOs) in peels of C. reticulata ‘Dahongpao’, a cultivar of C. reticulata Blanco (mandarin) from the same source was investigated by gas chromatography-mass spectrometry (GC–MS) compiled with Automated Mass Spectral Deconvolution & Identification System (AMDIS). Peels were collected once a month during July-December in 2011 from the identical area. The components in EOs were identified by their purified mass spectra and Kováts retention indices (RIs), in sum, 87 compounds were identified. A total of 67, 77, 66, 51, 46, and 59 compounds representing 89.856%, 92.151%, 97.881%, 97.738%, 97.229%, and 97.897% of their total relative contents, respectively in the EOs from six samples with the delay collected time were discerned. The EOs from six samples varied considerably both qualitatively and quantitatively. Chemical changeability of six samples differed with each other to some degree. 14 biomarkers were established, 8 of them like d-limonene, γ-terpinene, linalool, decanal, α-sinensal, thymol, nhexadecanoic acid and tricosane are more highlighted. Based on the contents of 3 biomarkers like dlimonene, γ-terpinene and linalool, the chemotype as limonene/γ-terpinene/linalool (55.111– 77.659%/6.579-7.841%/1.197-7.773%) was differentiated for these EOs from C. reticulata ‘Dahongpao’. The study manifests AMDIS can efficiently enhance the accuracy of quantitative and qualitative analyses by extracting information from total ion chromatograms (TICs). The results have some meanings to clarify the similarities and differences in bioactive compounds as volatile oils of Pericarpium Citri Reticulatae Viride (PCRV) and Pericarpium Citri Reticulatae (PCR) and to expound the scientific essence of “One material used as two kinds of Chinese materia medica (CMM)” such as PCRV and PCR in the end.

Author (s) Details

Jian Wang
College of Chinese Medicine, Chongqing Medical University, Chongqing 400016, China.

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