The objective of this research was to perform a quantitative and comparative analysis of physical and chemical characteristics and antioxidant activity in organic and conventional carrot (Daucus carota), green pepper (Capsicum annuum) and lettuce (Lactuca sativa). Five representative samples of each conventional vegetables, certified organic and non-certified organic vegetables were gotten from farms and supermarkets in the city of Rio de Janeiro, Brazil. All samples were underwent the following analyzes: reducing sugars, total sugars, ºBrix, vitamin C, density, acidity, antioxidant activity and phenolic compounds content. Data were submitted to analysis of variance (ANOVA) and the means compared by Tukey’s test at 5% probability. The result shows that the organic carrot showed higher acidity (0.11 g% citric acid) and total sugar (5.68 g%) than those found in standard samples and certified organic ones (p<0.05). Regarding the density analysis and total soluble solids, there was no statistical difference between carrots, green peppers and lettuce from all types (p>0.05). It was observed that the vitamin C levels in carrot samples levels had no significant difference between the different forms of production (p>0.05). Conventional lettuce and certified organic pepper showed higher vitamin C than the other samples (p<0.05). The antioxidant activity of the samples was analyzed by the capacity to reduce the DPPH (1,1-diphenyl-2-picryl- hydrazyl) radical, in which carrot and conventional pepper showed lower antioxidant activity (p<0.05) when compared to organic samples. There were no significant differences among the different forms of production in the lettuce samples (p>0.05). Carrot and green pepper, with seal certification or not, showed higher capacity to reduce DPPH than the conventional ones, this suggests that the form of cultivation has a direct relationship with the nutritional values of the vegetables.

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