News Release on Metabolism Research: September-2018

Our intestinal microbiome influences metabolism — through the immune system

Research tells the North American nation that the commensal or “good” microorganism that inhabits our intestines facilitate to manage our metabolism. a replacement study in fruit flies, revealed solstice in Cell Metabolism, shows one stunning method they are doing this.

The study, diode by Paula Watnick, MD, Ph.D., of the Division of Infectious Diseases at state capital Children’s Hospital, reveals that innate immune pathways, best called our initial line of defense against microorganism infection, have a facet job that is equally vital. [1]

Differential phosphatidic acid metabolism in barley leaves and roots induced by chilling temperature

Phosphatidic acid (PA) is a crucial bioactive lipide that mediates chilling responses in barley. Modifications within the lipid composition of cellular membranes throughout chilling square measure essential to keep up their integrity and fluidness. First, we tend to investigate the molecular species of PA gift in leaves and roots by ESI-MS/MS, to judge the modifications that occur in response to chilling. we tend to incontestible that PA pools in leaves dissent from PA carboxylic acid composition in roots. Compared with plants grown at 25 °C, the short-term and long-term chilling for 3 h and 36 h at 4 °C not produced significant changes in PA molecular species. [2]

Model‐Based Identification of Cell‐Cycle Dependent Metabolism and Putative Autocrine Effects in Antibody Producing CHO Cell Culture

The understanding of cell‐cycle dependent population heterogeneities in mammalian cell culture and their influence on production rates are still limited. moreover, metabolic regulations arising from self‐expressed signal factors (autocrine/autoinhibitory factors) have been postulated in the past, but no determination of such effects have been made so far for fast‐growing production CHO cells in chemically defined media. during this work, a novel approach combining near‐physiological treatment of cells (including synchronization), population resolved mechanistic modeling and applied math analysis was developed to spot population inhomogeneities. Cell-cycle-dependent population dynamics and metabolic regulations due to a putative autocrine factor were examined and their impact on the metabolic rates and antibody production of near‐physiologically synchronized CHO DP‐12 cell cultures was determined. [3]

Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress

Gomisin N (GN), a lignan derived from Schisandra Chinensis, has been shown to possess inhibitor, anti-inflammatory drug, and antineoplastic properties. within the gift study, we have a tendency to investigated the protecting result of GN against ethanol-induced liver injury mistreatment in vivo and in vitro experiments. Histopathological examination unconcealed that GN administration to chronic-binge plant product exposure mice considerably reduced ethanol-induced internal organ steatosis through reducing lipogenesis organic phenomenon and increasing carboxylic acid chemical reaction organic phenomenon, and prevented liver injury by lowering the liquid body substance levels of aspartate transferase and amino acid transferase. Further, it considerably stifled hemoprotein P450 2E1 (CYP2E1) organic phenomenon and protein activity and increased inhibitor genes and glutathione level in internal organ tissues, that crystal rectifier to weakened internal organ malondialdehyde levels. It additionally down inflammation organic phenomenon. [4]

FUNCTIONALIZATION AND CONJUGATION AS DRUG METABOLISM PATHWAYS: INDIVIDUAL REACTIONS AND A LOOK AT ON COMPROMISING FACTORS: A REVIEW

Drug metabolism pathways are the chemical processes typically in 2 phases: phase I referred to as functionalization and clinical trial as conjugation. The reactions of phase I embody chemical reaction, reduction, and hydrolytic reactions; they introduce a purposeful cluster that builds the drug a lot of polar. clinical trial reactions are those within which associate endogenous compound reminiscent of glucuronic acid or glutathione mix with purposeful cluster derived from phase I to supply a extremely polar drug conjugate. These reactions represent a site wherever biochemists and pharmacologists collaborate within the search of latest drug entities (NDEs) so that they ar a basic understanding of the role of the chemistry within the biotransformation of drug within the body. [5]

Reference

[1] Our intestinal microbiome influences metabolism — through the immune system

Date: June 21, 2018, Source: Boston Children’s Hospital (web link)

[2] Differential phosphatidic acid metabolism in barley leaves and roots induced by chilling temperature

Margutti MP, Gaveglio VL, Reyna M, Pasquaré SJ, Racagni GE, Villasuso AL. Differential phosphatidic acid metabolism in barley leaves and roots induced by chilling temperature. Plant Physiology and Biochemistry. 2018 Sep 1. (web link)

[3] Model‐Based Identification of Cell‐Cycle Dependent Metabolism and Putative Autocrine Effects in Antibody Producing CHO Cell Culture

Möller J, Korte K, Pörtner R, Zeng AP, Jandt U. Model‐Based Identification of Cell‐Cycle Dependent Metabolism and Putative Autocrine Effects in Antibody Producing CHO Cell Culture. Biotechnology and Bioengineering. 2018 Sep 1. (web link)

[4] Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress

Nagappan A, Jung D, Kim JH, Lee H, Jung M. Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress. International Journal of Molecular Sciences. 2018 Sep 1;19(9):2601. (web link)

[5] FUNCTIONALIZATION AND CONJUGATION AS DRUG METABOLISM PATHWAYS: INDIVIDUAL REACTIONS AND A LOOK AT ON COMPROMISING FACTORS: A REVIEW

JUSTIN N. KABERA1,2*, ALLY R. MUSSA1, EDMOND SEMANA1, YANG FAN1 AND HE XIN1,3

1College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

2National Industrial Research and Development Agency, Kigali, Rwanda.

3Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin, China. (web link)