Ashwagandha, Tulsi, Mulethi, Awala, Shatavari, Gokharu, Arjun, Giloy, Safed musli, Kalimirchi, Haldi, Jaiphal can be used as an active ingredients and aqueous extract of Stevia rebaudiana as natural sweetener with nutraceutical in health dinks. The product can be developed by treating concentrates of each crude drug with purified water. TLC profile, HPTLC method development and validation can be carried out using Gallic acid as a standard. A new simple, sensitive, selective, precise and robust HPTLC method for analysis of Gallic acid in health drink can be developed. Precoated silica health drink aluminium plate 60F-254 (20 × 10 cm) with 200 µm thickness can be used as stationary phase while toluene: ethyl acetate: formic acid: ethanol (6: 4: 0.3: 0.4) system can be developed as a mobile phase. Spectrum analysis showed the same Rf values and spectrum pattern of standard and sample. The method can be validated by using accuracy, precision, linearity, robustness, ruggedness and recovery as applicable parameters. The developed method can be quite good and most sensitive for the present products. The unpleasant and bitter taste of the product can be masked by different concentrations of aqueous extract of Stevia. Sweetness potency can be determined by taste evaluation method. 1% Stevia extract may be sufficient to produce most palatable and acceptable sweet preparation.
Author (s) Details
Dr. V. R. Salunkhe
Department of Pharmacognosy, Rajarambapu College of Pharmacy, Kasegaon, Dist. Sangli, Maharastra, India.
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