High Throughput Transcriptome Analysis Reveals Repair of Genes Involved in Insulin Signalling Pathway in MIN6 Cells on Treatment with TG

High Throughput Transcriptome Analysis Reveals Repair of Genes Involved in Insulin Signalling Pathway in MIN6 Cells on Treatment with TG

Diabetes is a metabolic condition in which insulin resistance and hyperglycemia are classified, resulting in reduced glucose absorption. Numerous plant species have historically been used as natural therapies for the treatment of diabetes. A recognised antidiabetic medicinal plant is well developed in the traditional Indian ‘Ayurveda’ system among these Gymnema sylvestre. A TG triterpene glycoside fraction isolated and purified from Gymnema sylvestre ethanolic extract (EEGS) was investigated using in vitro methods for blood glucose regulation benifits. We already defined TG in our previous work as a mixture of triterpene glycosides; gymnemic acid I, IV, VII, and gymnemagenin. The objective of the present study was to investigate the impact of triterpene glycosides (TG) on the battery of insulin signalling targets by high-throughput MIN 6 cell gene profiling. Because of its insulin stimulatory function, transcriptome analysis showed the repair of genes in TG treated cells. The transcriptome findings were further confirmed by RT-PCR experiments on selected targets for stimulating insulin; Glut2, ppar-γ, Ins, Irs, Akt1 and Pdx1. TG displayed enhanced expression of insulin stimulating genes at a concentration of 50 μg (range 1-2.5 folds relative to untreated cells), supporting TG’s ameliorative pharmacological impact on insulin resistance. In addition, TG (at 50 μg concentration) showed an increase in calcium uptake in MIN6 cells with an increased time period of up to 10 min, suggesting possible activation of gated calcium channels by voltage. The transcriptome study of TG treated MIN6 cells at 50 μg concentration was thus confirmed by in vitro studies. TG’s increase in insulin gene expression compared very well with the improvement in transcription factor expression responsible for the regulation of insulin gene expression. In the improvement of insulin resistance in diabetic patients, TG may therefore be considered as a healthy possible functional phytoingredient.

Author (s) Details

Dr. Rashmi S. Shenoy
Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India and Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India.

Dr. H. K. Manonmani
Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India and Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India.

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