Latest Research on Lactobacillus Plantarum: Dec – 2019

Complete genome sequence of Lactobacillus plantarum WCFS1

The 3,308,274-bp sequence of the body of true bacteria plantarum strain WCFS1, one colony isolate of strain NCIMB8826 that was originally isolated from human secretion, has been determined, and contains three,052 expected protein-encoding genes. supposed biological functions can be assigned  to a pair of,120 (70%) of the anticipated proteins. according to the classification of L. plantarum as a facultative heterofermentative carboxylic acid bacteria, the ordering encodes all enzymes needed for the metabolism and phosphoketolase pathways, all of that seem to belong to the category of doubtless extremely expressed genes during this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes an outsized pyruvate-dissipating potential, resulting in numerous end-products of fermentation. L. [1]

Isolation and characterization of the pseudocatalase of Lactobacillus plantarum.

The nonheme, or pseudo, enzyme of true bacteria plantarum has been pure to homogeneity. This catalyst is pink in targeted solutions and includes a mass of 172,000  4,000. it’s composed of six noncovalently associated subunits of mass twenty eight,300  600. This pseudocatalase contains one.12  0.37 atoms of metal per monetary unit. Fe, Co, Cu, and Zn, if gift the least bit, were at but zero.1 atom per monetary unit. At pH 7.0 and at twenty five degrees C the metric linear unit for H2O2 is 250 millimeter and also the turnover variety at Vm was three.9 x 10(5) metric weight unitecule|mole|metric weight unit|weight unit} of H2O2 per mol of catalyst per s. The optical spectrum of pseudocatalase is analogous to it of the metal SOD and indicates the presence of Mn(III) within the resting catalyst. [2]

Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1

Lactobacillus plantarum F1 and L. brevis OG1 isolated from Nigerian soured food merchandise, created bacteriocins that had broad spectrum of inhibition against each unhealthful, food spoilage organisms and numerous carboxylic acid bacterium.  The check organisms exhibited activities of 6400 and 3200 AU/ml severally against E. coli NCTC10418 and Enterococcus faecalis EF1, however failed to inhibit candida ATCC10231 and enterics sp. UCH15. Comparison of the antimicrobial spectra and characterization of the 2 bacteriocins weren’t identical. Bacteriocin created by L. brevis OG1 was the foremost heat stable at 121°C for sixty min, whereas that of L. plantarum F1 was stable at 121°C for ten min. The bacteriocins created by the check isolates maintained full stability once storage for sixty days at – 20°C; partial stability once storage for a hundred and twenty days at 4°C; whereas activity wasn’t detected once storage for eighty to a hundred and twenty days at 37°C. Bacteriocin created by L. brevis OG1 was stable at pH vary of two.0 to 8.0 while, that of L. [3]

Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract

Host diet may be a major determinant of the composition and performance of the enteric microbiome. Less understood is that the importance of diet on eaten strains with probiotic significance. we have a tendency to investigated the population dynamics of exogenous microorganism} plantarum and its interactions with enteric bacteria in mice undergoing switches between high-fat, high-sugar (HFHSD) and low-fat, plant-polysaccharide made (LFPPD) diets. The survival and persistence of eaten L. plantarum WCFS1 was considerably improved throughout mouse consumption of HFHSD and was negatively related to the numbers of native true bacteria species. [4]

Properties of Alpha-amylase of Lactobacillus plantarum Isolated from Cassava Waste Samples

The look for numerous industrial enzymes as well as starch degrading enzymes has received an excellent deal of attention for his or her perceived technological and economic advantages. Alpha-amylase has been made from giant sorts of organisms as well as morbific ones that don’t seem to be typically thought to be Safe (GRAS). during this study, a complete of Thirty 9 (39) carboxylic acid bacterium were isolated from the various cassava waste samples (peels, cassava waste water, fibre and soil). The isolates were screened for his or her ability to supply α-amylase on starch agar. The chemical process activity of the chosen isolates was firm exploitation starch-iodine complicated whereas the enzymes were characterised supported parameters like pH scale, temperature, substrate concentration, and metal ions. Upon starch chemical reaction, 29.9% of the workplace isolates incontestible chemical process properties with L. plantarum having the very best prevalence. [5]

Reference

[1] Kleerebezem, M., Boekhorst, J., van Kranenburg, R., Molenaar, D., Kuipers, O.P., Leer, R., Tarchini, R., Peters, S.A., Sandbrink, H.M., Fiers, M.W. and Stiekema, W., 2003. Complete genome sequence of Lactobacillus plantarum WCFS1. Proceedings of the National Academy of Sciences, 100(4), (Web Link)

[2] Kono, Y. and Fridovich, I., 1983. Isolation and characterization of the pseudocatalase of Lactobacillus plantarum. Journal of Biological Chemistry, 258(10), (Web Link)

[3] Ogunbanwo, S.T., Sanni, A.I. and Onilude, A.A., 2003. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. African Journal of Biotechnology, 2(8), (Web Link)

[4] Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
Xiaochen Yin, Bokyung Lee, Jose Zaragoza & Maria L. Marco
Scientific Reports volume 7, (Web Link)

[5] Abiodun Onilude, A., Solomon Ayinla, G. and Eluehike, C. (2017) “Properties of Alpha-amylase of Lactobacillus plantarum Isolated from Cassava Waste Samples”, Biotechnology Journal International, 19(1), (Web Link)