News Update on Pseudomonas Research: Dec – 2019

News Update on Pseudomonas Research: Dec – 2019

News Update on Pseudomonas Research: Dec – 2019

Characteristics of Pseudomonas solanacearum

A collection of 185 isolates of ring rot bacteria has been classified into 4 biotypes consistent with their capacity to oxidize 3 disaccharides (lactose, maltose and cellobiose) and three hexose alcohols (mannitol, sorbitol and dulcitol). Biotype 2 which oxidized the disaccharides but not the hexose alcohols appears to possess a restricted host range; it had been obtained solely from two plant hosts, potato and tomato, whereas biotype 1, which oxidized neither group of carbohydrates, and biotype 3 which oxidized both, were obtained from a diversity of plant hosts. A bacteriophage isolated from material infected with biotype 2 showed a high degree of specificity for isolates of biotype 2. this known distribution of the 4 biotypes is given and their relationship to host range is discussed. A neotype culture for Ps. solanacearum is proposed. [1]

Antimicrobial susceptibility of Pseudomonas aeruginosa: results of a UK survey and evaluation of the British Society for Antimicrobial Chemotherapy disc susceptibility test

A survey was conducted in 1999, first to work out the prevalence of antibiotic resistance among clinical isolates of Pseudomonas aeruginosa within the united kingdom and secondly to see whether the use of the standardized British Society for Antimicrobial Chemotherapy (BSAC) disc testing method improved the accuracy of routine susceptibility testing for this organism. Twenty-five hospitals were each asked to collect up to 100 consecutive, clinically significant isolates of P. aeruginosa and to see their susceptibility to amikacin, gentamicin, ceftazidime, imipenem, meropenem, ciprofloxacin, piperacillin and piperacillin/tazobactam using the new BSAC disc method. an entire of 2194 isolate reports were available for analysis and 10% of the isolates represented, plus those with unusual resistances, were re-tested centrally for control purposes. [2]

Chromium reduction in Pseudomonas putida

Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was related to soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells. [3]

Biodegradation of Vulcanized SBR: A Comparison between Bacillus subtilis, Pseudomonas aeruginosa and Streptomyces sp

Rubber residues present harmful impacts on health and environment, besides wasting valuable and large amounts of rubber. Biological recycling technique is concentrated here to attenuate this problem. A comparison of the biodegradation effect caused by Bacillus subtilis, Pseudomonas aeruginosa, and Streptomyces sp., separately, on vulcanized SBR-rubber during 4 weeks is reported. The surface and molecular analyses were studied by FTIR-ATR, TGA, DSC, TC and SEM/EDS, additionally to the contact angle and crosslinking tests. B. subtilis, P. aeruginosa, and Streptomyces sp. evoked after 4 weeks a loss in v-SBR crosslinks by 17.15, 10.68 and 43.39% and also within the contact angle with water by 14.10, 12.86 and 15.71%, respectively., if compared to regulate samples. FTIR findings indicate that the polymeric chain has been partially consumed causing C-C bonds scission indicating the biodegradation and bio-devulcanization phenomena. [4]

blaOXA-48 Carbapenem Resistant Pseudomonas aeruginosa Clinical Isolates in Sudan

Carbapenems are the pis aller of antimicrobial therapy, resistance to antibiotics specially carbapenems poses a worldwide problem which will have devastating consequences on the community.

We investigated 67 multidrug resistant clinical isolates of Pseudomonas aeruginosa for the presence of the blaoxa-48 gene using real time PCR.

The isolates were obtained from Three major hospitals in Khartoum state Sudan; Army hospital, Khartoum hospital and therefore the national Ribat university teaching hospital, from December 2015 to January 2017.

Out of 67 samples, 22.4% were positive for the blaoxa-48, 46.3% were immune to Imipenem and 32.8% of the samples were immune to Meropenem. [5]

Reference

[1] Hayward, A.C., 1964. Characteristics of Pseudomonas solanacearum. Journal of Applied Bacteriology, 27(2), (Web Link)

[2] Henwood, C.J., Livermore, D.M., James, D., Warner, M. and Pseudomonas Study Group, T., 2001. Antimicrobial susceptibility of Pseudomonas aeruginosa: results of a UK survey and evaluation of the British Society for Antimicrobial Chemotherapy disc susceptibility test. Journal of Antimicrobial Chemotherapy, 47(6), (Web Link)

[3] Ishibashi, Y., Cervantes, C. and Silver, S., 1990. Chromium reduction in Pseudomonas putida. Appl. Environ. Microbiol., 56(7), (Web Link)

[4] Biodegradation of Vulcanized SBR: A Comparison between Bacillus subtilis, Pseudomonas aeruginosa and Streptomyces sp
Mostafa Galal Aboelkheir, Priscilla Braga Bedor, Selma Gomes Leite, Kaushik Pal, Romildo Dias Toledo Filho & Fernando Gomes de Souza Jr.
Scientific Reports volume 9, (Web Link)

[5] Elnour Rahma Mohamed, S., Alobied, A., Mohamed Hussien, W. and Ibrahim Saeed, M. (2018) “blaOXA-48 Carbapenem Resistant Pseudomonas aeruginosa Clinical Isolates in Sudan”, Journal of Advances in Microbiology, 10(4), (Web Link)

Editor 251News

leave a comment

Create Account



Log In Your Account