News Update on Salmonella Research: Jan – 2020

Salmonella Nomenclature

Salmonellosis may be a major explanation for bacterial enteric illness in both humans and animals. annually an estimated 1.4 million cases of salmonellosis occur among humans within the us (15). In approximately 35,000 of those cases,Salmonella isolates are serotyped by public health laboratories and therefore the results are electronically transmitted to the Centers for Disease Control and Prevention (CDC). This information is employed by local and state health departments and CDC to watch local, regional, and national trends in human salmonellosis and to spot possible outbreaks of salmonellosis (1, 5). Over the past 25 years, the National Salmonella closed-circuit television has provided valuable information on the incidence of human salmonellosis within the us and trends in specific serotypes. [1]

Extra-intestinal manifestations of salmonella infections.

While salmonellosis is usually considered to affect primarily the alimentary canal , infection at other sites may occur, producing characteristic clinical syndromes. We reviewed cases from our institutions and therefore the literature on focal manifestations of salmonella infections. within the past, most extra-intestinal salmonella infections were caused by S. choleraesuis; however, we found S. typhimurium to be the predominant serotype. The deathrate for patients in our series was considerably less than the speed described for focal infections in other reviews. this might partially flow from to lower proportion of infections thanks to S. choleraesuis, improved microbiologic and diagnostic techniques, increased use of ampicillin, and improved surgical techniques. Salmonella endocarditis usually occurs in patients with preexisting heart condition . [2]

Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test

We describe during this paper the overall methods for using the Salmonella/microsome test as a mutagenesis screening system. The methods described include the quality plate test, the utilization and storage of the bacterial tester strains, preparation and use of the liver homogenates (S/sub 9/), and therefore the methods of inducing the rats for elevated microsomal enzyme activity. the appliance of this test system to screening large numbers of compounds, and therefore the interpretation of test results is additionally discussed. [3]

Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection

MicroRNAs (miRNAs) are increasingly recognized for his or her role in infection by bacterial pathogens, although the effect of every individual miRNA remains largely unknown. Here, we used a comparative genome-wide microscopy-based functional screening approach to spot miRNAs controlling infection by two bacterial pathogens—Salmonella enterica serovar Typhimurium and Shigella flexneri. Despite the similarities between these pathogens, we found infections to be controlled by largely non-overlapping subsets of miRNAs, seemingly reflecting different requirements prompted by their distinct intracellular lifestyles. [4]

Risk Factors Associated with Salmonella Contamination of Chicken Carcases in Traditional Slaughterhouses in Morocco

Aim: the target of this study was to spot the danger factors for Salmonella spp. contamination of Moroccan chicken carcases during slaughtering. Sixty four traditional slaughter houses were studied from October 2014 to June 2016 in Ouarzazate (Morocco).

Methodology: A questionnaire was submitted to the slaughterers and samples of breast skin were taken to assess the Salmonella spp. status of chicken carcases. [5]

Reference

[1] Brenner, F.W., Villar, R.G., Angulo, F.J., Tauxe, R. and Swaminathan, B., 2000. Salmonella nomenclature. Journal of clinical microbiology, 38(7), (Web Link)

[2] Cohen, J.I., Bartlett, J.A. and Corey, G.R., 1987. Extra-intestinal manifestations of salmonella infections. Medicine, 66(5), (Web Link)

[3] Ames, B.N., McCann, J. and Yamasaki, E., 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutation Research/Environmental Mutagenesis and Related Subjects, 31(6), (Web Link)

[4] Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection
Carmen Aguilar, Ana Rita Cruz, Ines Rodrigues Lopes, Claire Maudet, Ushasree Sunkavalli, Ricardo Jorge Silva, Malvika Sharan, Clivia Lisowski, Sara Zaldívar-López, Juan José Garrido, Mauro Giacca, Miguel Mano & Ana Eulalio
Nature Microbiology volume 5, (Web Link)

[5] Chaiba, A. and Filali, F. R. (2017) “Risk Factors Associated with Salmonella Contamination of Chicken Carcases in Traditional Slaughterhouses in Morocco”, Microbiology Research Journal International, 19(6), (Web Link)