The current research proposes a mass spectrometric patch-clamp system with a capillary that performs both local potential registration at the cell membrane and analyte suction at the same time. This paper compares the capabilities of the novel approach proposed with those of existing methods such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or “ion microscopy,” live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, and so on. We also look at approaches to increase the informativeness of these methodologies, with a particular focus on the trend of rising analysis complexity. We suggest a method for increasing the efficacy of cell trapping to the capillary during MS-path-clamp, as well as providing laser surface ionisation with laser trapping and tweezing of cells using the capillary as a waveguide. As an optional route of further development of the complex of analytical techniques coming from the MS variation of patch-clamp, the aforesaid system can be combined with the microcolumn separation system or capillary electrophoresis. In its current form, the concept of “MS-patch-clamp” is more than a research method; it’s a fundamental approach that can be used to a wide range of detection, ionisation, and desorption procedures, as well as numerous methods of analyte supply.
Author (S) Details
Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
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