News Update on Crude Extracts Research: June – 2019

Phytochemical Studies And Comparative Efficacy Of The Crude Extracts Of Some Haemostatic Plants In Edo And Delta States Of Nigeria

Leaves of Ageratum conyzoides (L), Alchornea cordifolia (Schym and Thonn) Muel. Arg, Aspilia africana (Pers.) C. D. Adams, Baphia nitida (Lodd), Chromolaena odorata (L) K. R., Landophia owariensis (P. Beauv) and sap of rosid dicot genus curcas (L) used historically to arrest injury in contemporary cuts were relatively investigated phytochemically and their ability to precipitate and coagulate plasma. Saponins and tannins were the foremost abundant  compounds in these plants whereas flavoids were the smallest amount. Crude binary compound extracts of alkaloids, flavonoids, tannins and saponins from these plants precipitated and coagulated plasma among deadlines of four to one hundred twenty seconds (for precipitation) and fifteen to 1500 seconds (for coagulation). Results from factor II temporal arrangement showed that A. afriana was the foremost efficacious haemostatic plant followed by L. owariensis, and L. curcas the smallest amount. Some similarities in their chemical composition established a scientific basis for common usage in ancient medication. [1]

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples

Lipidomics, when genetic science and genetics, may be a fresh and apace increasing analysis field that studies cellular macromoleculeomes and therefore the structure hierarchy of lipid and supermolecule constituents mediating life processes. Lipidomics is greatly expedited by recent advances in, and novel applications of, electrospray ionization mass spectrographic analysis (ESI/MS). during this review, we’ll target the advances in ESI/MS, that have expedited the event of firearm lipidomics and therefore the utility of intrasource separation as associate degree sanctioning strategy for utilization of second mass spectrographic analysis in shotgun lipidomics of biological samples. The principles and experimental details of the intrasource separation approach are going to be extensively mentioned. different ESI/MS approaches towards the quantitative analyses of world cellular macromoleculeomes directly from crude lipid extracts of biological samples also will be reviewed and compared. Multiple samples of macromoleculeomic analyses from crude lipid extracts using these approaches are going to be given to point out the ability of ESI/MS techniques in lipidomics. Currently, fashionable society is stricken by the sequelae of lipid‐related diseases. it’s our hope that the mixing of those advances in multiple disciplines can change state the event of lipidomics, and such development can result in enhancements in medical specialty and medicine, which can ultimately lead to the extended longevity and an improved quality of life for mankind. © 2004 Wiley Periodicals, Inc., Mass verbal description Rev 24:367–412, 2005 [2]

Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria.

Different crude extracts of Nigella sativa were tested for antimicrobial effectiveness against totally different microorganism isolates. These isolates comprised sixteen gramnegative and vi grampositive representatives. They showed multiple resistance against antibiotics, specially the gramnegative ones. Crude extracts of flower spit showed a promising result against a number of the check organisms. the foremost effective extracts were the crude organic compound and water extracts. Gramnegative isolates were affected quite the grampositive ones. [3]

Affimer reagents as tools in diagnosing plant virus diseases

Plant viruses will cause devastating losses to agriculture and are thus a significant threat to food security. The speedy identification of virally-infected crops permitting containment is crucial to limit such threats, however plant infective agent diseases will be very difficult to diagnose. a perfect technique for virus designation would be a tool which might be enforced simply within the field. Such devices need a binding chemical agent that’s specific for the virus of interest. we have a tendency to selected to research the utilization of Affimer reagents, artificial binding proteins and a model virus Cowpea Mosaic virus (CPMV) empty virus like particles (eVLPs). CPMV-eVLP mimic the morphology of wild-type (WT) CPMV however lack any infectious genomic material so don’t have biocontainment problems. we’ve made associate degreed refined an Affimer chemical agent selected for its ability to bind to CPMV-eVLP and have shown that the chosen Affimer conjointly specifically binds to WT CPMV. we’ve made a three.4 Å structure of WT CPMV absolute to the Affimer mistreatment cryo-electron research. Finally, we’ve shown that this Affimer is capable of faithfully police investigation the virus in crude extracts of CPMV-infected leaves and might thus kind the idea for the longer term development of diagnostic tests [4]

Phytochemical Screening of Crude Extracts of Bridelia micrantha

In Kenya, twenty two million individuals area unit in danger of protozoal infection, seventieth of them area unit in rural areas, and most of those individuals use ancient plant-based medicines to treat protozoal infection. the utilization of natural product-derived medication and medicines from alternative sources in combating protozoal infection has but been Janus-faced with many challenges, as well as the emergence of drug resistance parasites, thereby creating several of the primary line medication like antimalarial (CQ) not economical. The aim of this study was to work out the phytochemical properties of Bridelia micrantha accustomed treat protozoal infection among the Digo community in Kenya. The crude extracts were obtained exploitation solvent, ester, and wood alcohol. A Shimadzu 8400 FT-IR was accustomed confirm the purposeful teams gift within the crude extracts. From the results obtained, varied purposeful teams characteristic of phenolics, tannins, saponins, alkaloids, flavonoids, and glycosides were found to be gift. A quantitative assay was performed exploitation Shimadzu 6200 UV-VIS photometer. the overall phenolics and flavonoids were found to be highest in barks with a amount of zero.84±0.14 mg/g and of zero.86±0.03 mg/g severally whereas glucoside content was found to be highest in leaves with a amount of twelve.66±0.23 mg/g. organic compound content, on the opposite hand, was found to be highest in roots with a amount of 313.44±0.05 mg/g. [5]


[1] Obadoni, B.O. and Ochuko, P.O., 2002. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of pure and applied sciences, 8(2), pp.203-208. (Web Link)

[2] Han, X. and Gross, R.W., 2005. Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass spectrometry reviews, 24(3), pp.367-412. (Web Link)

[3] Morsi, N.M., 2000. Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria. Acta Microbiologica Polonica, 49(1), pp.63-74. (Web Link)

[4] Affimer reagents as tools in diagnosing plant virus diseases

Emma L. Hesketh, Christian Tiede, Hope Adamson, Thomas L. Adams, Matthew J. Byrne, Yulia Meshcheriakova, Inga Kruse, Michael J. McPherson, George P. Lomonossoff, Darren C. Tomlinson & Neil A. Ranson

Scientific Reportsvolume 9, Article number: 7524 (2019) (Web Link)

[5] Mburu, C., G. Kareru, P., Kipyegon, C., S. Madivoli, E., G. Maina, E., Kairigo, P. K., K. Kimani, P. and M. Marikah, D. (2016) “Phytochemical Screening of Crude Extracts of Bridelia micrantha”, European Journal of Medicinal Plants, 16(1), pp. 1-7. doi: 10.9734/EJMP/2016/26649. (Web Link)