In the report, two PCR based molecular gravestones like RAPD and ISSR has been used to distinguish different populations of Zingiberaceae. Molecular description of Hedychium coronarium from four different populace in Odisha using nine ISSR and fifteen RAPD markers to fast and efficiently determine the nearness of species and hybrids. A dendrogram was assembled through sequential agglomerative hierarchial and nested (SHAN) grouping and un-weighted pair group order with arithmetic mean (UPGMA) reasoning using Jaccard’s correspondence coefficient of combined gravestones using this particular species. Two main clusters were found that is, cluster-I (Malkangiri-1, Phulabani-1, Phulabani-3, Malkangiri-2, Khurda-1, Khurda-2, Khurda-3, Angul-3, Angul-1, Angul-2 and Phulabani-2) and cluster-II (Malkangiri-3). The clustering pattern also told moreover in consideration of genetic similarity middle from two points germplasms collected from four different domains population. The brimming potential of this technique will be realised for the labeling and tagging of important novel genes in earlier unexplored taxa, facilitating the bettering of desired Zingiberaceae taxa. The findings hopeful of immense enough meaning for complementing the strategies of preservation and characterization of these main taxa of Zingibearceae following modern biotechnological approach. The competence of ISSR in clonal loyalty assessment on Allium and Aloe was settled successfully.

Author(s) Details:

Reena Parida,
Molecular Biology and Genetic Engineering Laboratory, Centre for Biotechnology, Siksha ‘O’ Anusandhan University, Bhubaneswar, Odisha, India.

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Keywords: Hedychium coronarium, ISSR, RAPD, molecular characterization