Recent Advancements of High-Performance Thin Layer Chromatography Method for Identification and Quantification of Oleanolic Acid in the Roots of Achyranthes aspera Linn.

Recent Advancements of High-Performance Thin Layer Chromatography Method for Identification and Quantification of Oleanolic Acid in the Roots of Achyranthes aspera Linn.

Aim: There is a link between biological activities and secondary metabolite presence in plants. A chromatographic method using HPTLC for the detection and quantification of oleanolic acid from the roots of Achyranthes aspera Linn is being developed and validated as part of the present research work. Methods: In current research, the External Standard Method is used for quantification analysis. In quantitative analysis, it ensures precision and accuracy and is particularly suited for HPTLC studies. Chromatography was performed on 250 μm thick aluminium silica gel 60F254 HPTLC solvent toluene plates: ethyl acetate: methanol: acetone 14:4:1:1 (v / v / v / v) as the mobile step. Derivatization was performed, scanned and quantified at 540 nm with Anisaldehyde sulphuric acid.
RESULTS: In plant roots, the concentration of oleanolic acid was found to be 1,277ng / μg. The statistical analysis proved that the approach developed is acceptable and specific. Conclusion: For routine quality control analysis and quantitative determination of oleanolic acid from A, the developed HPTLC technique can be used. Linn’s Aspera. In the range of 0.20-0.60 μg / μl, oleanolic acid has been found to be linear. An alternative quantification technique of this marker constituent was generated to ensure the identity and quality of the selected plant, taking into account the broad therapeutic applications of oleanolic acid. The HPTLC method for the quantification of oleanolic acid from the roots of Achyranthes aspera Linn is sensitive, precise, and reproducible.

Author(s) Details

Dr. Aparna A. Saraf
Department of Botany, The Institute of Science, Mumbai, Maharashtra, India.

Dr. Aruna C. Samant
Department of Botany, The Institute of Science, Mumbai, Maharashtra, India.


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