Wild Grape Fruits Extracted by Different Solvents: Phytochemicals and Biological Activities

Wild Grape Fruits Extracted by Different Solvents: Phytochemicals and Biological Activities

The aim of this study was to look into the phytochemical, antioxidant, and antibacterial activities of different coloured wild grape fruits extracted with different solvents such as water, methanol, and ethanol. Folin-Ciocalteu and colorimetric aluminium chloride assays were used to assess total phenolic (TPC) and flavonoid (TFC) content, while 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant strength (FRAP) assays were used to determine antioxidant activities. The extracts’ antibacterial activity against infective bacteria was also examined using the agar well diffusion process. In comparison to other extracts, methanolic extracts had the highest TPC content, especially in green extract. The highest TFC was found in the ethanolic extracts of red and black fruits, while the highest TFC was found in the green extract using methanol. As compared to other solvents of the same hue, methanolic extracts of all colours had the lowest IC50 values. The methanolic extract of green has the lowest IC50 values, indicating that it has the most strong antioxidant activity. The obtained results showed a strong correlation with the FRAP values. In contrast to the methanolic extract, the ethanolic extract showed comparable antioxidant activity. The antibacterial activity of a methanolic extract of green fruits was strong. All ethanolic extracts inhibited selected bacteria in a similar way, but none of the water extracts displayed any activity. Both extracts had MICs and MBCs in the 250-500 g/mL range. The obtained results showed that the phytochemicals and biological activities of the wild grape were primarily influenced by extraction solvents and different colours of large grape fruits.

Author (s) Details

Boonsod Yardpiroon
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.

Sangdee Aphidech
Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.

Srihanam Prasong
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.

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